Publications

and community-acquired infections. It has been reported that the clinical isolates of P.aeruginosa are difficult to treat because of their virulence factors and antibiotics resistances.The aim of present study was to screen the antibiotic resistance patterns and the prevalenceof virulence factor genes in a set of Pseudomonas aeruginosa isolated from Ogbomoso, andto determine whether a correlation exists between the prevalence of virulence factors andantibiotic resistance of P. aeruginosa. A total of 100 P. aeruginosa isolates were collectedfrom various types of clinical specimens. Antimicrobial susceptibility testing was performedusing the Kirby-bauer method. In addition, PCR assays were used for screening four virulenceencoding genes (OPRL, LasB, PLCH and ToxA). The results showed that OPRL (79%) and LasB(62%) were the most frequent virulence genes in P. aeruginosa strains, followed by PLCH (41%)and ToxA (35%). The highest resistance was detected towards Piperacillin (42%) andTetracycline (42%). Moderate rate of resistance (12-39%) were detected towards the otherantibiotics. The virulent factors identified in this study provide valuable informationregarding the prevalence of resistance genes of P. aeruginosa isolates in Ogbomoso, Nigeriaand their potential impact on treatments that exploit the unique physiology of the pathogen.This will be useful for the health workers to improve infection control measures and toestablish a surveillance system.