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HIV/AIDS is a major concern in all countries and all races of the world and it is a bigger problem in developingcountries and especially in Sub-Saharan Africa and in Uganda. The prevalence of HIV among adults aged 15 to 64in Uganda was 6.2% according to Uganda Population-Based HIV Impact Survey. This study was designed todetermine the prevalence of HIV/AIDS among motorcyclists in Lwamata town council Kiboga district. This studyemployed a cross-section descriptive study design. It was conducted in Lwamata town council Kiboga district among119 participants (motorcyclists) who were selected using a convenience sampling technique. A questionnaire wasused to collect data and all collected data was coded, entered, and analyzed using SPSS version 25. A greater numberof participants were aged 18-34 years (56.3%), by religion many were Catholics 45(37.8%), the majority 54(45.4%)were single, and by education, many 84(70.6%) were of secondary level. According to figure 1 below, 2.2(18.5%)tested HIV/AIDS positive while 97(81.5%) tested negative. Thus the prevalence of HIV/AIDS among studyparticipants was 18.5%. The factor significantly associated with HIV/AIDS test results was the number of partners(3 Vs 1). Participants with one partner were 56% less likely to be HIV positive compared to those with 3 partners,(aOR=0.442, 95% CI=0.213-0.922). The prevalence of HIV/AIDs among motorcyclists in Lwamata town councilKiboga district is high which was significantly associated with a number of partners that is having more than onepartner increases the chances of getting HIV/AIDs.