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Diabetic nephropathy has been incriminated to be the most important cause of end stage renal diseases and death inpatients with diabetes mellitus. This study was aimed at evaluating β2 microglobulin, cystatin c and creatinine inpatient with type 2 diabetes mellitus. A total of 40 subjects comprising of 20 diabetic patient and 20 healthy subjectswere recruited randomly for the study, and their blood sugar level was determined. Five milliliters (5 ml) of bloodwas then collected into the vaccutainer tube with minimal stasis. The blood was allowed to clot at room temperature,and serum separated and harvested into a clean dry well labeled sample bottles following centrifugation at 3000 rpmfor 5 minutes. The sample was stored in a freezer at -20oc, prior to use. β2 microglobulin and cystatin c wasdetermined using ELISA techniques while and creatinine was determined using Jaffe-slot Alkaline picratecolometricmethod. Results were presented in mean ± standard deviation (SD). All data obtained in the study were analyzedusing student t-test (spss.20) and Pearson correlation coefficent. The level of significance was set at p < 0.05. Therewas a significant increase (p=0.000) in the mean value of β2microglobulin in Diabetic patients (2.52±0.22 mg/L)when compared to controls (1.54±0.23 mg/L). The mean value of Cystatin C was significantly increased (p=0.009)in Diabetic patients (1.10±0.87 mg/ml) when compared to Controls (0.79±0.18 mg/ml). The mean value ofCreatinine was significantly increased (p=0.023) in Diabetic patients (1.10±0.87 mg/ml) when compared to Controls(0.79±0.18 mg/ml). There was a non-significant negative correlation of serum β2microglobulin with serum cystatinC and Creatinine (r= -0.638, p=0.247 and r=-0.115, p=0.853). Diabetes mellitus is associated with increased level ofβ2Microglobulin, Cystatin C and Creatinine. The changes observed in these parameters are critical as this maysuggest their use as early risk predictors of diabetic nephropathy in patients diagnosed of diabetes mellitus