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There is currently little information on the acceptability of male circumcision in Uganda. This study investigatedthe acceptability of male circumcision among mothers in the Kiryandongo district with male children. A crosssectionalsurvey was conducted among a convenience sample of 100 women attending a reproductive health clinicat the outpatient department (OPD) waiting area in Kiryandongo district hospital in Uganda between February andApril 2018. Of the 100 invited eligible participants, the majority of women were Christians (78%), (18%) wereMuslims, and (4%) were Pentecostal. About 60% of respondents had at least attained primary education (28%) hadsecondary education 9% had tertiary education and only 3% had no education at all. After women were informedabout the risks and benefits of male circumcision, the majority of women with uncircumcised children 81% said theywould definitely circumcise their children if the procedure were offered in a safe hospital setting, free of charge anda smaller number (7%) said they would probably consider the procedure. Only (1%) said that they would definitelyprobably not consider male circumcision, and (9%) were unsure. Since male circumcision has been found to decreasethe risk of HIV infection. Among men, it is important to determine its acceptability as a potential HIV preventionstrategy at even an early stage in Uganda. This study found male circumcision to be highly acceptable among abroad range of mothers with male children in Kiryandongo district Uganda. Further studies of acceptability amongfathers and other populations are warranted.